Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite hole.
Identifieur interne : 000264 ( Main/Exploration ); précédent : 000263; suivant : 000265Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite hole.
Auteurs : Brent C. Christner [États-Unis] ; Brian H. Kvitko ; John N. ReeveSource :
- Extremophiles : life under extreme conditions [ 1431-0651 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN ribosomique (composition chimique), ADN ribosomique (génétique), ARN ribosomique 16S (génétique), ARN ribosomique 18S (génétique), Bactéries (génétique), Cellules eucaryotes (MeSH), Clonage moléculaire (MeSH), Phylogenèse (MeSH), Phénomènes physiologiques bactériens (MeSH), Proteobacteria (génétique), Régions antarctiques (MeSH), Température (MeSH), Évolution moléculaire (MeSH).
- MESH :
- composition chimique : ADN ribosomique.
- génétique : ADN ribosomique, ARN ribosomique 16S, ARN ribosomique 18S, Bactéries, Proteobacteria.
- Cellules eucaryotes, Clonage moléculaire, Phylogenèse, Phénomènes physiologiques bactériens, Régions antarctiques, Température, Évolution moléculaire.
English descriptors
- KwdEn :
- Antarctic Regions (MeSH), Bacteria (genetics), Bacterial Physiological Phenomena (MeSH), Cloning, Molecular (MeSH), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Eukaryotic Cells (MeSH), Evolution, Molecular (MeSH), Phylogeny (MeSH), Proteobacteria (genetics), RNA, Ribosomal, 16S (genetics), RNA, Ribosomal, 18S (genetics), Temperature (MeSH).
- MESH :
- chemical , chemistry : DNA, Ribosomal.
- chemical , genetics : DNA, Ribosomal, RNA, Ribosomal, 16S, RNA, Ribosomal, 18S.
- geographic : Antarctic Regions.
- genetics : Bacteria, Proteobacteria.
- Bacterial Physiological Phenomena, Cloning, Molecular, Eukaryotic Cells, Evolution, Molecular, Phylogeny, Temperature.
Abstract
Inhabitants of a cryoconite hole formed in the Canada Glacier in the McMurdo Dry Valley region of Antarctica have been isolated and identified by small subunit (16S/18S) rDNA amplification, cloning, and sequencing. The sequences obtained revealed the presence of members of eight bacterial lineages (Acidobacterium, Actinobacteria, Cyanobacteria, Cytophagales, Gemmimonas, Planctomycetes, Proteobacteria, and Verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (Choiromyces), ciliate (Spathidium), and green algal (Pleurastrium) Eukarya. Bacterial recovery was approximately 20-fold higher at 4 degrees C and 15 degrees C than at 22 degrees C, and obligately psychrophilic bacteria were identified and isolated. Several of the rDNA molecules amplified from isolates and directly from cryoconite DNA preparations had sequences similar to rDNA molecules of species present in adjacent lake ice and microbial mat environments. This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments.
DOI: 10.1007/s00792-002-0309-0
PubMed: 12768448
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Reeve</name></author></analytic><series><title level="j">Extremophiles : life under extreme conditions</title><idno type="ISSN">1431-0651</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antarctic Regions (MeSH)</term><term>Bacteria (genetics)</term><term>Bacterial Physiological Phenomena (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Eukaryotic Cells (MeSH)</term><term>Evolution, Molecular (MeSH)</term><term>Phylogeny (MeSH)</term><term>Proteobacteria (genetics)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>RNA, Ribosomal, 18S (genetics)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ribosomique (composition chimique)</term><term>ADN ribosomique (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>ARN ribosomique 18S (génétique)</term><term>Bactéries (génétique)</term><term>Cellules eucaryotes (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phénomènes physiologiques bactériens (MeSH)</term><term>Proteobacteria (génétique)</term><term>Régions antarctiques (MeSH)</term><term>Température (MeSH)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term><term>RNA, Ribosomal, 18S</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Antarctic Regions</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN ribosomique</term><term>ARN ribosomique 16S</term><term>ARN ribosomique 18S</term><term>Bactéries</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Physiological Phenomena</term><term>Cloning, Molecular</term><term>Eukaryotic Cells</term><term>Evolution, Molecular</term><term>Phylogeny</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules eucaryotes</term><term>Clonage moléculaire</term><term>Phylogenèse</term><term>Phénomènes physiologiques bactériens</term><term>Régions antarctiques</term><term>Température</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Inhabitants of a cryoconite hole formed in the Canada Glacier in the McMurdo Dry Valley region of Antarctica have been isolated and identified by small subunit (16S/18S) rDNA amplification, cloning, and sequencing. The sequences obtained revealed the presence of members of eight bacterial lineages (Acidobacterium, Actinobacteria, Cyanobacteria, Cytophagales, Gemmimonas, Planctomycetes, Proteobacteria, and Verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (Choiromyces), ciliate (Spathidium), and green algal (Pleurastrium) Eukarya. Bacterial recovery was approximately 20-fold higher at 4 degrees C and 15 degrees C than at 22 degrees C, and obligately psychrophilic bacteria were identified and isolated. Several of the rDNA molecules amplified from isolates and directly from cryoconite DNA preparations had sequences similar to rDNA molecules of species present in adjacent lake ice and microbial mat environments. This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12768448</PMID><DateCompleted><Year>2004</Year><Month>02</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1431-0651</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>3</Issue><PubDate><Year>2003</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Extremophiles : life under extreme conditions</Title></Journal><ArticleTitle>Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite hole.</ArticleTitle><Pagination><MedlinePgn>177-83</MedlinePgn></Pagination><Abstract><AbstractText>Inhabitants of a cryoconite hole formed in the Canada Glacier in the McMurdo Dry Valley region of Antarctica have been isolated and identified by small subunit (16S/18S) rDNA amplification, cloning, and sequencing. The sequences obtained revealed the presence of members of eight bacterial lineages (Acidobacterium, Actinobacteria, Cyanobacteria, Cytophagales, Gemmimonas, Planctomycetes, Proteobacteria, and Verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (Choiromyces), ciliate (Spathidium), and green algal (Pleurastrium) Eukarya. Bacterial recovery was approximately 20-fold higher at 4 degrees C and 15 degrees C than at 22 degrees C, and obligately psychrophilic bacteria were identified and isolated. Several of the rDNA molecules amplified from isolates and directly from cryoconite DNA preparations had sequences similar to rDNA molecules of species present in adjacent lake ice and microbial mat environments. This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Christner</LastName><ForeName>Brent C</ForeName><Initials>BC</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kvitko</LastName><ForeName>Brian H</ForeName><Initials>BH</Initials><Suffix>2nd</Suffix></Author><Author ValidYN="Y"><LastName>Reeve</LastName><ForeName>John N</ForeName><Initials>JN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate 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Sep;35(8):499-503</Citation><ArticleIdList><ArticleId IdType="pubmed">11539025</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><noCountry><name sortKey="Kvitko, Brian H" sort="Kvitko, Brian H" uniqKey="Kvitko B" first="Brian H" last="Kvitko">Brian H. Kvitko</name><name sortKey="Reeve, John N" sort="Reeve, John N" uniqKey="Reeve J" first="John N" last="Reeve">John N. Reeve</name></noCountry><country name="États-Unis"><region name="Ohio"><name sortKey="Christner, Brent C" sort="Christner, Brent C" uniqKey="Christner B" first="Brent C" last="Christner">Brent C. Christner</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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